Assessment of the fungal isolates interactions using laboratory culture technique with brown lentil seed germination using Egyptian vermicompost suspension

Document Type : Original Article

Authors

1 Botany and microbilogy, Faculty of Science, Helwan University, Cairo

2 Biotechnology,faculty of science,helwan university,Cairo

3 BIotechnology,faculty of science,helwan university,Cairo

4 biotechnology,faculty of science,helwan university,Cairo

Abstract

This study focuses on the interaction among vermicompost fungi and vermicompost's ability to enhance seed germination by cultivating brown lentil seeds through using filter paper and pertri-dishes technique. Vermicompost also prepared using wastes materials and earthworms, then used for fungal isolation and lentil seed germination in the suspended form. The interaction among vermicompost fungi was observed during direct isolations using the serial dilution method and then using pure culture dual plate's plans. At the same time, brown lentil seed germination was occurred using vermicompost suspension and measuring seed germination percentage method. The results revealed that most isolated microorganisms were fungal isolates which used for study interaction among vermicompost fungi varied from neutral interaction to negative interaction; brown lentil seeds achieved a high germination percentage (80%) using vermicompost suspension compared to a positive and negative control. The overall results confirmed that the vermicomposting fungi play an essential role as biological control and growth-promoting factor.

Keywords

African Journal of Biological Sciences

Articles in Press, Accepted Manuscript
Available Online from 15 February 2021

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