Assessment of the fungal isolates interactions by laboratory culture technique with brown lentil seeds germination using vermicompost suspension

Document Type : Original Article

Authors

1 Botany and microbilogy, Faculty of Science, Helwan University, Cairo

2 Department of Biotechnology, Faculty of Science, Helwan Univ., Cairo, Egypt

Abstract

This study focuses on the interactions among vermicompost fungi and vermicompost's ability to enhance seed germination by cultivatingbrown lentil seeds through using filter paper and pertri-dishes technique. Vermicompost prepared by using waste materials and earthworms was used for fungal isolation and lentil seed germination in the suspended form. The interaction among vermicompost fungi was observed during direct isolations using the serial dilution method and then using pure culture dual plate's plans. At the same time, brown lentil seed germination was occurred using vermicompost suspension and measuring seed germination percentage method. The results revealed that most isolated microorganisms were fungi.  Interaction among vermicompost fungi varied from neutral to negative interaction. The brown lentil seedsachieved a high germination percentage (80%) using vermicompost suspension compared to a positive and negative control. The overall results confirmed that the vermicomposting fungi play an essential role as biological control and growth-promoting factor.

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