Molecular genetic studies on Atropa belladonna L. under effect of laser radiation and salicylic acid on Atropine, Scopolamine and Hyoscyamine by tissue culture techniques

Document Type : Original Article

Authors

1 Agric. Botany- Department, Genetics, Faculty of Agriculture, Al-Azhar University Cairo, Egypt.

2 Botany Department, National Research Centre, Dokki, Cairo, Egypt.

Abstract

The most important commercial source of pharmaceutical tropane alkaloids such as Atropine, Scopolamine, and Hyoscyamine is Atropa belladonna. The current study is to investigate both effects of different helium-neon laser doses (0.0, 10, 15, 20, 25 and 30 J cm-2) and exposure conditions on callus fresh weight and apply biotechnological tools as alternative approach for increasing the accumulation of active agents (atropine) of A. belladonna plant using abiotic elicitors salicylic acid precursor feeding. After treatment they were analyzed by A photograph illustrating the banding profile produced by the twelve ISSR primers DNA in the six laser radiations (0.0, 10, 15, 20, 25 and 30 J cm-2) was in volved and HPLC technique was applied to determine Atropine, Scopolamine and Hyoscyamine contents. The results showed that the highest callus fresh weight, callus dry weight was achieved by callus derived from (leaves and stem) explants, which was cultured on (MS) medium supplemented with different combinations among different concentrations of growth regulators as NAA, 2,4-D, Kin and BAP (at 0.5 and 1.0 mg/l). On the other hand all treatment of callus tissue had affected the content of medically active constituents of Atropa belladonna. This study suggests a more efficient method for commercially large-scale production of Atropine, Scopolamine, and Hyoscyamine by A. belladonna plants.

Keywords

African Journal of Biological Sciences

Articles in Press, Accepted Manuscript
Available Online from 25 July 2022

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