Using green coffee and cinnamon extracts for Regulation of the proliferation signaling and inflammatory events during treating breast cancer

Document Type : Original Article

Authors

1 Department of moeculer bilolgy, Genetic Engineering and Biotechnology Research Institute, University of Sadat City

2 Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt

Abstract

According to NCI, one-third of all female cancer diagnoses in Egypt are due to breast carcinoma. This research investigates the use of low-cost, ecologically-friendly plant extracts (green coffee and cinnamon) dissolved in Ethanol for the treatment of breast cancer. Many women prefer natural medicines and conventional ones to manage their illness, reduce sideeffects from medication, or slow the progression of the disease. The potential effects of these plant extracts on MCF-7 cells were analyzed using MTT assay, qRT-PCR, and ELISA. The results showed that cells were substantially destroyed by green coffee extract. The cells exposed to green coffee, like those exposed to cinnamon extracts, generated considerably more LDH. Research compared the effects of cinnamon and green coffee extracts on MCF-7 cells and found that the latter had a greater impact on apoptosis. The proteins p53 and caspase-3, as well as the ERK signaling cascade, were influenced by the green coffee components.RAF/MEK/ERK pathway in MCF-7 cells was inhibited. By altering critical apoptotic signaling pathways, green coffee may exhibit anti-cancer effects and promote apoptosis in breast cancer cells. The average levels of IL-1α and IL-1β, measured by ELISA, were found to have risen dramatically after 24-hours. While IL-6 and IL-8 mean concentration shows a steady state. Finally, green coffee extract was shown to inhibit the production of pro-inflammatory cytokines.

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