• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Peer Review Process
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
African Journal of Biological Sciences
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 17 (2021)
Volume Volume 16 (2020)
Volume Volume 15 (2019)
Issue Issue 1
Volume Volume 14 (2018)
Volume Volume 13 (2017)
Abushiba, M., El-Sherbiny, G., Moghannem, S., Kalaba, M., El- Shatoury, E., Saeed, A. (2019). Enhancement of Antibiotics activity by microbially synthesized silver nanoparticles. African Journal of Biological Sciences, 15(1), 137-153. doi: 10.21608/ajbs.2019.64008
Mahmoud Abushiba; Gamal El-Sherbiny; Saad Moghannem; Mohamed Kalaba; Einas El- Shatoury; Ali Saeed. "Enhancement of Antibiotics activity by microbially synthesized silver nanoparticles". African Journal of Biological Sciences, 15, 1, 2019, 137-153. doi: 10.21608/ajbs.2019.64008
Abushiba, M., El-Sherbiny, G., Moghannem, S., Kalaba, M., El- Shatoury, E., Saeed, A. (2019). 'Enhancement of Antibiotics activity by microbially synthesized silver nanoparticles', African Journal of Biological Sciences, 15(1), pp. 137-153. doi: 10.21608/ajbs.2019.64008
Abushiba, M., El-Sherbiny, G., Moghannem, S., Kalaba, M., El- Shatoury, E., Saeed, A. Enhancement of Antibiotics activity by microbially synthesized silver nanoparticles. African Journal of Biological Sciences, 2019; 15(1): 137-153. doi: 10.21608/ajbs.2019.64008

Enhancement of Antibiotics activity by microbially synthesized silver nanoparticles

Article 11, Volume 15, Issue 1, 2019, Page 137-153  XML PDF (613.67 K)
Document Type: Original Article
DOI: 10.21608/ajbs.2019.64008
Authors
Mahmoud Abushiba1; Gamal El-Sherbiny2; Saad Moghannem3; Mohamed Kalaba2; Einas El- Shatoury4; Ali Saeed5
1Department of Biology, Faculty of Science, Azzaytuna University, Tarhuna, Libya
2Department of Botany and Microbiology, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt
3Department of Botany and Microbiology, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
4Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt
5-Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt
Abstract
Biological synthesis of nanoparticles is a promising approach that is comparatively inexpensive and eco-friendly. Among various microorganisms, actinobacteria have been found to be efficient in the synthesis of nanomaterials. In thisstudy, extracellular biosynthesis of silver nanoparticles (Ag-NPs) was investigated by mixing silver nitrate with culture filtrate of Streptomyces rochei strain MFA-37.The biosynthesized Ag-NPs were characterized by UV–Vis spectrophotometric analysis, and showed a peak of absorbance at 440 nm. Fourier transform infrared (FTIR) analysis showed amines and amides that are responsible for the stabilization of Ag-NPs. The morphology and size of nanoparticles were determined using high-resolution transmission electron microscopy, XRD and DLS techniques which indicated well dispersed, spherical Ag-NPs sized 2–20 nm, and the zeta potential of AgNPs reached -20.5 mV. Enhancement of antibacterial activity of fourteen antibiotics was conducted by combining antibiotics with synthesized AgNPs. The synergistic activities of AgNPs combination with antibiotics were determined using disc diffusion against two multi-drug resistant Staphylococcus aureus SA-185 and SA-325 recovered from Libya. The highest synergistic effects were 6.11 and 6.37 folds for Penicillin followed by 6.11 and 4.44 folds for Ciprofloxacin and 6.11 and 3.69 folds for Erythromycin for MRSA-185 and MRSA-325 , respectively. This activity of AgNPs and antibiotics could be valuable for the manufacture of hybrid drugs to kill or inhibit the MRSA infection especially in case of superficial injuries.
Keywords
MRSA; Ag-NPs; antibiotics; synergistic effects
Statistics
Article View: 516
PDF Download: 132
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by NotionWave.